Oocyte meiotic spindle assembly and function aaron f. Previous studies have proposed that these meiotic spindles arise from rangtpmediated mt nucleation in the vicinity of chromatin. Pdf visualization and examination of the meiotic spindle in. The study of the meiotic spindle assembly checkpoint pathway has been mentioned in research publications which can be found using our bioinformatics tool below. Using polarized light microscopy, images of mii oocytes were captured after retrieval of oocytes in stimulated cycles at six time intervals in culture. Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and icsi outcomes. Tdm1 regulation determines the number of meiotic divisions. A key feature of meiosis is the reduction of the number of chromosomes, from two sets to one set, prior to fertilization. The best understood meiotic drive systems are the segregation distorter sd system in drosophila and the thaplotype in mouse.
Relationship between meiotic spindle location with regard to. Results show that selection of embryos based on zona pellucida and meiotic spindle imaging can significantly improve implantation and pregnancy rates. Spindle apparatus is a descriptor in the national library of medicines controlled vocabulary thesaurus, mesh medical subject headings. Moreover, the dose of fsh used for ovarian stimulation may affect the organization of. Visualization of the metaphase ii meiotic spindle in. Meiotic spindle size is a strong indicator of human oocyte. Architectural dynamics of the meiotic spindle revealed by.
The polscope system generates contrast to image the meiotic spindle on the basis of its birefringence. On the basis of spindle visualization or not, two groups were established. Advanced 4d tracking of meiotic spindle dynamic arivis ag. C weak visualization, d spindle on the 1stpolar body border and cytoplasm.
Pdf visualization and examination of the meiotic spindle. The double reflection is generated by the interaction of polarized light beam with the highly ordered assembly of microtubules building the bipolar spindle. Dec 18, 2006 the conserved localization of katanin at spindle poles of many species and the similarity of spindle length phenotypes in cv1 cells and c. The meiotic spindle assembly checkpoint pathway has been researched in relation to spindle assembly checkpoint, meiosis, spindle assembly, cell cycle, prophase. Successful cell division requires proper force generation and responses of the microtubulebased spindle. To assess possible effects on subcellular organization after cryopreservation, we compared vitrified and slowly frozen oocytes in terms of their postwarmthaw morphology, meiotic spindle configuration, and dna integrity. Therefore, cdca8 might also influence microfilaments assembly and regulate spindle the position. Female meiotic spindles in many organisms form in the absence of centrosomes, the organelle typically associated with microtubule mt nucleation. Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity. Relationship between visualization of meiotic spindle in. The generality of katanins relative role in the assembly of mitotic versus meiotic spindles. In support of this idea, meiotic spindle visualization reveals that these apparently mature oocytes first polar body extruded may still be at telophase i or prometaphase ii143 144 145 and even. Sumoylation regulates protein dynamics during meiotic.
Visualization of meiotic spindle and subsequent embryonic. This meiotic acceleration was detrimental to the fidelity of bivalent segregation, leading to high rates of homologue nondisjunction. In these studies, meiotic spindles could be detected in 61. Preliminary results, fertility and sterility on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. A sister chromatids grouped at the poles b individual chromosomes all at the cells center c an uninterrupted spindle array d the synaptonemal complex e tetrads all aligned at the cells center answer. Central spindle cls2 localisation is regulated by sumo. Katanin controls mitotic and meiotic spindle length journal. It is referred to as the mitotic spindle during mitosis, a process that produces genetically identical daughter cells, or the meiotic spindle during meiosis, a process that produces gametes with half the number of chromosomes of the parent cell. Relationship between meiotic spindles visualization and. This reduction in chromosome number is due to two cell divisions following a single round of dna replication. In summary, there was good analog between visualization of meiotic spindle and levels of oxidative stress markers as reliable predictors of oocyte assessment and embryo development.
The second type of cytological study of early development in bivalves concerns investigations in developmental biology andor cytology. Data of 83 ivficsiet cycle, including pregnancy results, and a total of 1033 oocytes examined. Dynamic maintenance of asymmetric meiotic spindle position through arp23complexdriven cytoplasmic streaming in mouse oocytes kexiyi1,jayr. Spindle rotation is indispensable for pbe, and microfilaments play a vital role in regulating meiotic spindle rotation zhu et al. Visualization and examination of the meiotic spindle in human. Meiotic spindle visualization in living human oocytes. Visualization of the metaphase ii meiotic spindle in living. Noninvasive imaging of the meiotic spindle of in vivo matured. The effect of detectabilitylocation of meiotic spindle to fertilization of human mii oocytes and outcome of clinical ivficsiet cycles was studied methods.
The aim of this study was to examine changes in meiotic spindle morphology over time to potentially optimize timing for icsi. Time course of meiotic spindle development in mii oocytes. To visualize and identify meiotic cells at metaphase with a microscope, what would you look for. High oxidative stress appears to inhibit meiotic spindle formation and subsequently affects embryo quality. The aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes. According to the metaanalysis, the results showed statistically significant higher fertilization rate p cleveland state university, cleveland, ohio, usa oregon institute of marine biology, university of oregon, charleston. Visualization of the metaphase ii meiotic spindle in living human oocytes using the polscope enables the prediction of embryonic developmental competence after icsi jeonghee moon1, changseop hyun, seokwon lee, weonyoung son, sanhyun yoon and jinho lim maria infertility hospital, seoul, korea. The meiotic spindle visualization was performed at 20. Sep 01, 2010 read zona pellucida birefringence and meiotic spindle visualization as predictors of icsi outcomes. The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. Read oocyte meioticstage specific differences in spindle depolymerization in response to temperature changes, fertility and sterility on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Apcfzr1 prevents nondisjunction in mouse oocytes by.
Jul 27, 2018 during meiotic drive, some chromosomes can bias their spindle orientation and thus be retained in the egg. But before we get into this, a bit of explanation about spindles themselves. Slaughter1,borisrubinstein1 androngli1,3,4 mature mammalian oocytes are poised for completing meiosis ii mii on fertilization by positioning the. Here, the authors find that this phenomenon can be driven by microtubule force. Visualization and examination of the meiotic spindle in human oocytes with polscope. Polarized light microscopy plm combined with an image processing software allows for noninvasive visualization of the meiotic spindle in the live oocyte. Valea,1 athe howard hughes medical institute and department of cellular and molecular pharmacology, university of california, san francisco, ca 94158. A computerassisted polarization microscopy system polscope has made it possible to analyse the meiotic spindle of oocytes subjected to intracytoplasmic sperm injection icsi without affecting their viability. Oocyte meioticstage specific differences in spindle. The effect of detectabilitylocation of meiotic spindle to fertilization of human mii oocytes and outcome of clinical ivficsiet cycles was studied. Besides chromosomes, the spindle apparatus is composed of hundreds of proteins. Ten trials fulfilled the inclusion criteria comparing invitro and clinical icsi outcomes with or without visualization of meiotic spindle in fresh and invivo matured oocytes. Pld2 regulates microtubule stability and spindle migration in mouse oocytes during meiotic division xiaoyu liu, xiaoyun liu, dandan chen, xiuying jiang, wei ma department of histology and embryology, school of basic medical sciences, capital medical university, beijing, china. However, recent observations made with the use of a novel orientation.
Human egg maturity assessment and its clinical application. During meiotic drive, some chromosomes can bias their spindle orientation and thus be retained in the egg. Neither the content nor the benchsci technology and processes for selection have been evaluated by us. Meiotic spindle assembly checkpoint pathway bioinformatics. Moreover, the dose of fsh used for ovarian stimulation may affect the organization of the oocyte meiotic spindle. Visualization and examination of the meiotic spindle in. Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes. Data of 83 ivficsiet cycle, including pregnancy results, and a total of 1033 oocytes. Polscope analysis of meiotic spindle changes in living.
The objective of this metaanalysis was to investigate the in. With its expertise on correlative light and electron microscopy clem, the laboratory of prof. Spindle assembly and function, like cytokinesis, is a very broad topic. Dec 01, 2017 the second type of cytological study of early development in bivalves concerns investigations in developmental biology andor cytology. According to the metaanalysis, the results showed statistically significant higher fertilization rate p meiotic spindle was viewed than when it was. At the time of icsi, polscope and associating software spindleview, usa was used to examine the. Article zona pellucida birefringence score and meiotic. Sep 27, 2007 the aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes.
Graph drawing is an area of mathematics and computer science combining methods from geometric graph theory and information visualization to derive twodimensional depictions of graphs arising from applications such as social network analysis, cartography, linguistics, and bioinformatics. Pld2 regulates microtubule stability and spindle migration in. We observed that fzr1 controlled the speed of spindle assembly in such a way that its loss accelerated bivalent biorientation and consequently the timing of sac satisfaction. The sd system involves the interaction of a mutant form of a nuclear transport protein with a simple repetitive sequence. Dynamic maintenance of asymmetric meiotic spindle position. The spindles of 4 in vivo matured, 105 in vitro matured oocytes were examined by polscope at the time of icsi. The conserved localization of katanin at spindle poles of many species and the similarity of spindlelength phenotypes in cv1 cells and c. The location of spindle relative to polar body positioned to 6 oclock was detected with polscope prior to icsi in oocytes. A kinesin14 motor activates neocentromeres to promote.
Relationship between meiotic spindle location with regard. Article relationship between visualization of meiotic spindle. Parallelaligned meiotic spindle microtubules are able to shift. Our particular interest is in the potential roles of actin filaments factin and the factinbased motor protein, myosin10, in spindle assembly and function. All meiotic spindle observations at different steps of the freezingthawing procedure were performed at room temperature. Another key protein during meiotic chromosome segregation is the clasp orthologue cls2 dumont et al. Spindle visualization by immunocytochemistry all chemicals used were purchased from sigma st louis, mo. To visualize and identify meiotic cells at metaphase with a.
This confirmed that there is a close relationship between the size of the meiotic spindle in human metaphase ii mii oocytes and embryo developmental potential after icsi. Clicking the images or links will redirect you to a website hosted by benchsci that provides thirdparty scientific content. The authors previous study indicated that the meiotic spindle characteristics are significantly associated with early or latecleaving embryos. Dna integrity of cryopreserved oocytes was not altered after the procedures, but vitrification was more effective than slow cooling, as shown by higher survival rate and. Assessment of oocyte quality in polycystic ovarian syndrome. Thomas mullerreichert at the tu dresden is committed to study the details of meiotic cell divisions and the function of the spindle apparatus during this process using the model organism c. Spindle tubulin and mtoc asymmetries may explain meiotic. If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Oocytes without spindle visualization were rotated by the injection microcapillary to exclude improper spindle alignment within the path of polarized light. Read zona pellucida birefringence and meiotic spindle visualization as predictors of icsi outcomes. It has been shown that the presence of a detectable birefringent meiotic spindle inside the oocyte cytoplasm of human metaphase ii mii prepared for icsi is an indicator of oocyte. Author summary meiosis is a fundamental process for sexually reproducing organisms that creates genetic diversity within populations. Oct 14, 2007 bipolarity of the meiotic spindle, required for proper chromosome segregation, is maintained throughout cell division despite rapid microtubule turnover. Augmin promotes meiotic spindle formation and bipolarity in.
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